Journal: Journal of Biomedical Materials Research. Part a

Article Title: Investigation on the antimicrobial properties of cerium‐doped bioactive glasses

doi: 10.1002/jbm.a.37289

Figure Lengend Snippet: Residual charge of live Escherichia coli on the BGs surfaces after 24 hr incubation at 30°C. Inoculum was diluted in NB/500 medium supplemented (green bars) or not (yellow bars) with 200 mM phosphate buffer (pH 7.0). Controls were incubated in the absence of BGs. The reported data (enumeration in LB plates) are means ± standard deviations of three independent experiments

Article Snippet: The antimicrobial activity of the Ce‐BGs was first investigated toward the Gram−negative reference strain E. coli ATCC 11229.

Techniques: Incubation

Journal: Journal of Biomedical Materials Research. Part a

Article Title: Investigation on the antimicrobial properties of cerium‐doped bioactive glasses

doi: 10.1002/jbm.a.37289

Figure Lengend Snippet: Charge [Log(CFU/cm 2 )] of microorganisms on the glass surface after 24 hr incubation at 30°C. Inocula were resuspended in the proper medium containing 200 mM PB (pH 7.0). All the Ce‐BGs were tested and compared with controls. The reported data are means ± standard deviations of three independent experiments. No significant difference between the H and K series and among controls and Ce‐BGs with different cerium amounts were observed (test‐t and ANOVA, p > .05)

Article Snippet: The antimicrobial activity of the Ce‐BGs was first investigated toward the Gram−negative reference strain E. coli ATCC 11229.

Techniques: Incubation, Control

Journal: Applied and Environmental Microbiology

Article Title: Rubber Oxygenase and Latex Clearing Protein Cleave Rubber to Different Products and Use Different Cleavage Mechanisms

doi: 10.1128/AEM.01271-14

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Cells were harvested (4°C) by centrifugation, and Lcp was purified from cell-free culture fluid as described below. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristic(s) a Source or reference Strains E. coli JM109 Plasmid storage XL1-Blue QuikChange transformation strain Stratagene Xanthomonas sp. 35Y Growth on poly( cis -1,4-isoprene) latex, clearing zone formation 16 35Y-CM Cm r mutant of 35Y 18 35Y Δ roxA-attB (SN3727) Chromosomal deletion of roxA in Xanthomonas sp. 35Y-CM, attB at former roxA site; no clearing zone formation on latex agar 4 35Y Δ roxA-attB pNH1- roxA-attP in chromosome (SN4230) Expression of RoxA from rhamnose promoter in Xanthomonas sp. 35Y-CM; Km r Cm r ; clearing zone formation in the presence of rhamnose plus latex 4 35Y Δ roxA-attB pNH1- lcp-attP in chromosome (SN5343) Expression of Lcp from rhamnose promoter in Xanthomonas sp. 35Y-CM; Km r Cm r ; positive fuchsin staining on latex overlay agar This study Plasmids pMK:: lcp (SN5314) GeneArt vector, source of lcp ; Km r This study pUC9:: lcp (SN5339) Cloning vector for lcp ; Ap r This study pNH1- roxA-attP (SN4230) Coding sequence of roxA under rhamnose promoter control, attP site; Km r 4 pNH1- lcp-attP (SN5341) Coding sequence of lcp under rhamnose promoter control, attP site; Km r This study Open in a separate window a Cm r , chloramphenicol resistance; Ap r , ampicillin resistance; Km r , kanamycin resistance.

Techniques: Plasmid Preparation, Transformation Assay, Mutagenesis, Expressing, Staining, Clone Assay, Sequencing

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: AFM images of bacterial lawns prepared with ATCC 19606 (panels A, C and E) and 19606R (panels B, D and F) at mid-logarithmic phase. Large scans (panels A and B; 20 × 20 μm) have been presented as height images which provide a reflection of true topographical data by describing changes in sample height; in this manner tall features are illustrated by bright regions, while dark regions portray areas of low sample height. Magnified surface plots (panels C and D; 10 × 10 μm) were constructed from height images, while cross-sections of the 10 × 10 μm plots are illustrated in panel E and F.

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Construct

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: Mean RMS-roughness (nm) ± SD of A. baumannii ATCC 19606 and 19606R at mid-logarithmic and stationary phase, and in response to treatment with 32 mgL −1 colistin (mid-logarithmic cells). Measurements were determined for five separately prepared bacterial lawns from AFM height images (10 × 10 μm).

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: High magnification AFM amplitude images (1 × 1 μm) of ATCC 19606 (panels A and B) and 19606R (panels C and D) at mid-logarithmic phase, illustrating untreated cells (panels A and C), and cells treated for 20 min with colistin 32 mgL−1 (panels B and D).

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: AFM images of bacterial lawns prepared with ATCC 19606 (panels A, C and E) and 19606R (panels B, D and F) at mid-logarithmic phase treated with 32 mgL−1 colistin, presented as: (i) 20 × 20 μm height images (panels A and B); (ii) magnified 10 × 10 μm surface plots, constructed from height images (panels C and D); and, (iii) cross-sections of the 10 × 10 μm surface plots (panels E and F).

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques: Construct

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: Change in contact angle (panels A and B, mean ± SD) and droplet volume (panels C and D, mean ± SD) illustrated as a function of time over ~12 sec for ATCC 19606 (panels A and C) and 19606R (panels B and D) at mid-logarithmic and stationary phase, and in response to colistin 32 mgL−1 (mid-logarithmic cells).

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Journal of applied microbiology

Article Title: Cell surface hydrophobicity of colistin-susceptible versus -resistant Acinetobacter baumannii determined by contact angles: methodological considerations and implications

doi: 10.1111/j.1365-2672.2012.05337.x

Figure Lengend Snippet: Contact angles on bacterial lawns of untreated colistin-susceptible and -resistant A. baumannii , at mid-logarithmic and stationary phase, and in response to colistin 32 mgL −1 (mid-logarithmic cells). Measurements obtained at the initial time-point (t = 0.66 sec) and final time-point (t ~12 sec) are presented.

Article Snippet: Reference strain ATCC 19606 (colistin MIC 1 mgL −1 ) was purchased from the American Type Culture Collection (Manassas, VA, USA).

Techniques:

Journal: Applied and Environmental Microbiology

Article Title: Heterologous Overexpression and Characterization of a Flavoprotein-Cytochrome c Complex Fructose Dehydrogenase of Gluconobacter japonicus NBRC3260

doi: 10.1128/AEM.03152-12

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Kanamycin and ampicillin were used at final concentrations of 50 μg ml −1 and 250 μg ml −1 , respectively. table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description Source or reference a Strains Escherichia coli DH5α F − endA1 hsdR17 (r k − m k + ) supE44 thi-1 λ − recAl gyrA96 relA1 deoR Δ( lacZYA-argF )U169 φ80d lacZ ΔM15 10 HB101 F − thi-1 hsdS20 (r B m B ) supE44 recA13 ara14 leuB6 proA2 lacY1 galK2 rpsL20 (Str r xyl-5 mtl-1 λ − ) 34 Gluconobacter japonicus NBRC3260 Wild type NBRC Gluconobacter oxydans NBRC12528 Wild type NBRC Δ adhA mutant NBRC12528 Δ adhA ::Km r 9 ATCC 621H Wild type ATCC Plasmids pKR2013 Plasmid mediates plasmid transfer; Km r 11 pBBR1MCS-4 Broad-host-range plasmid; mob Ap r 35 pSHO8 pBBR1MCS-4, a 0.7-kb fragment of a putative promoter region of the adhAB gene of G. oxydans 621H This study pSHO12 pSHO8, a 3.8-kb fragment of the fdhSCL genes of G. japonicus NBRC3260 This study pSHO13 pSHO8, a 3.7-kb fragment of the fdh ATG SCL genes This study pSHO16 pSHO8, a 2.4-kb fragment of the fdh ATG SL genes (in-frame deletion of fdhC ) This study Open in a separate window a The URL addresses of NBRC and ATCC are http://www.nbrc.nite.go.jp/ and http://www.atcc.org/ , respectively.

Techniques: Plasmid Preparation, Mutagenesis

Journal:

Article Title: Production of Optically Pure d -Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 †

doi: 10.1128/AEM.69.1.399-407.2003

Figure Lengend Snippet: E. coli strains and plasmids used in this study

Article Snippet: Broth cultures were grown in M9 medium containing either 1% glucose (tube experiments and seed cultures) or 5% glucose (pH-controlled fermentors). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains W3110 Wild type ATCC 27325 TOP10F′ lacI q (episome) Invitrogen DH5α Δ lacZ M15 recA Bethesda Research Laboratory S17-1 thi pro recA hsdR RP4-2- tet ::Mu aphA ::Tn Z λ pir 40 BW25113 lacI q rrnB Δ lacZ hsdR Δ araBAD Δ rhaBAD 16 AH218 Δ( focA-pflB ):: FRT-kan-FRT This study SE1706 Δ frdBC zid ::Tn 10 34 TC20 Δ adhE :: FRT-tet-FRT This study SZ31 W3110, Δ( focA-pflB ):: FRT-kan-FRT P1(AH218) × W3110 This study SZ32 W3110, Δ( focA-pflB ):: FRT This study SZ34 W3110, Δ( frdBC zid )::Tn 10 P1(SE1706) × W3110 This study SZ35 W3110, Δ( focA-pflB ):: FRT Δ frdBC zid ::Tn 10 P1(SE1706) × SZ32 This study SZ37 W3110, Δ frdBC This study SZ40 W3110, Δ( focA-pflB ):: FRT Δ frdBC This study SZ57 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT-tet-FRT P1(TC20) × SZ40 This study SZ58 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT This study SZ61 W3110, ackA :: FRT-tet-FRT This study SZ62 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT-tet-FRT P1(SZ61) × SZ58 This study SZ63 W3110, Δ focA-pflB :: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT This study Plasmids pCR2.1-TOPO bla kan , TOPO TA cloning vector Invitrogen pFT-A bla flp temperature-conditional replicon 37 pKD4 bla FRT-kan-FRT 16 pKD46 bla γ β exo (red recombinase), temperature-conditional replicon 16 pLOI2065 bla FRT-tet-FRT This study pLOI2224 kan , integration vector with conditional R6K replicon 31 pLOI2302 pUC19 containing Asc I linkers inserted into blunt Nde I and Sap I sites 46 pLOI2372 bla ackA This study pLOI2373 bla ackA :: FRT-tet-FRT This study pLOI2375 bla ackA :: FRT-tet-FRT conditional R6K replicon This study pLOI2803 bla kan Δ adhE :: FRT-tet-FRT This study Open in a separate window E. coli strains and plasmids used in this study Genetic methods.

Techniques: Plasmid Preparation, TA Cloning

Journal:

Article Title: Production of Optically Pure d -Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 †

doi: 10.1128/AEM.69.1.399-407.2003

Figure Lengend Snippet: Fermentation of 5% glucose by W3110 and derivatives of this strain. (A) W3110 (wild type); (B) SZ40; (C) SZ58; (D) SZ63; (E) SZ58 supplemented with 10 mM acetate; (F) SZ58 with 8 h of (initial) aeration. Symbols: •, cell mass; □, glucose concentration; ▪, lactate concentration; ▴, succinate concentration; ○, ethanol concentration; ▵, acetate; ∗, formate concentration. OD550, optical density at 550 nm.

Article Snippet: Broth cultures were grown in M9 medium containing either 1% glucose (tube experiments and seed cultures) or 5% glucose (pH-controlled fermentors). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains W3110 Wild type ATCC 27325 TOP10F′ lacI q (episome) Invitrogen DH5α Δ lacZ M15 recA Bethesda Research Laboratory S17-1 thi pro recA hsdR RP4-2- tet ::Mu aphA ::Tn Z λ pir 40 BW25113 lacI q rrnB Δ lacZ hsdR Δ araBAD Δ rhaBAD 16 AH218 Δ( focA-pflB ):: FRT-kan-FRT This study SE1706 Δ frdBC zid ::Tn 10 34 TC20 Δ adhE :: FRT-tet-FRT This study SZ31 W3110, Δ( focA-pflB ):: FRT-kan-FRT P1(AH218) × W3110 This study SZ32 W3110, Δ( focA-pflB ):: FRT This study SZ34 W3110, Δ( frdBC zid )::Tn 10 P1(SE1706) × W3110 This study SZ35 W3110, Δ( focA-pflB ):: FRT Δ frdBC zid ::Tn 10 P1(SE1706) × SZ32 This study SZ37 W3110, Δ frdBC This study SZ40 W3110, Δ( focA-pflB ):: FRT Δ frdBC This study SZ57 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT-tet-FRT P1(TC20) × SZ40 This study SZ58 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT This study SZ61 W3110, ackA :: FRT-tet-FRT This study SZ62 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT-tet-FRT P1(SZ61) × SZ58 This study SZ63 W3110, Δ focA-pflB :: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT This study Plasmids pCR2.1-TOPO bla kan , TOPO TA cloning vector Invitrogen pFT-A bla flp temperature-conditional replicon 37 pKD4 bla FRT-kan-FRT 16 pKD46 bla γ β exo (red recombinase), temperature-conditional replicon 16 pLOI2065 bla FRT-tet-FRT This study pLOI2224 kan , integration vector with conditional R6K replicon 31 pLOI2302 pUC19 containing Asc I linkers inserted into blunt Nde I and Sap I sites 46 pLOI2372 bla ackA This study pLOI2373 bla ackA :: FRT-tet-FRT This study pLOI2375 bla ackA :: FRT-tet-FRT conditional R6K replicon This study pLOI2803 bla kan Δ adhE :: FRT-tet-FRT This study Open in a separate window E. coli strains and plasmids used in this study Genetic methods.

Techniques: Concentration Assay

Journal:

Article Title: Production of Optically Pure d -Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 †

doi: 10.1128/AEM.69.1.399-407.2003

Figure Lengend Snippet: Fermentation products from glucose a

Article Snippet: Broth cultures were grown in M9 medium containing either 1% glucose (tube experiments and seed cultures) or 5% glucose (pH-controlled fermentors). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains W3110 Wild type ATCC 27325 TOP10F′ lacI q (episome) Invitrogen DH5α Δ lacZ M15 recA Bethesda Research Laboratory S17-1 thi pro recA hsdR RP4-2- tet ::Mu aphA ::Tn Z λ pir 40 BW25113 lacI q rrnB Δ lacZ hsdR Δ araBAD Δ rhaBAD 16 AH218 Δ( focA-pflB ):: FRT-kan-FRT This study SE1706 Δ frdBC zid ::Tn 10 34 TC20 Δ adhE :: FRT-tet-FRT This study SZ31 W3110, Δ( focA-pflB ):: FRT-kan-FRT P1(AH218) × W3110 This study SZ32 W3110, Δ( focA-pflB ):: FRT This study SZ34 W3110, Δ( frdBC zid )::Tn 10 P1(SE1706) × W3110 This study SZ35 W3110, Δ( focA-pflB ):: FRT Δ frdBC zid ::Tn 10 P1(SE1706) × SZ32 This study SZ37 W3110, Δ frdBC This study SZ40 W3110, Δ( focA-pflB ):: FRT Δ frdBC This study SZ57 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT-tet-FRT P1(TC20) × SZ40 This study SZ58 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT This study SZ61 W3110, ackA :: FRT-tet-FRT This study SZ62 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT-tet-FRT P1(SZ61) × SZ58 This study SZ63 W3110, Δ focA-pflB :: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT This study Plasmids pCR2.1-TOPO bla kan , TOPO TA cloning vector Invitrogen pFT-A bla flp temperature-conditional replicon 37 pKD4 bla FRT-kan-FRT 16 pKD46 bla γ β exo (red recombinase), temperature-conditional replicon 16 pLOI2065 bla FRT-tet-FRT This study pLOI2224 kan , integration vector with conditional R6K replicon 31 pLOI2302 pUC19 containing Asc I linkers inserted into blunt Nde I and Sap I sites 46 pLOI2372 bla ackA This study pLOI2373 bla ackA :: FRT-tet-FRT This study pLOI2375 bla ackA :: FRT-tet-FRT conditional R6K replicon This study pLOI2803 bla kan Δ adhE :: FRT-tet-FRT This study Open in a separate window E. coli strains and plasmids used in this study Genetic methods.

Techniques:

Journal:

Article Title: Production of Optically Pure d -Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 †

doi: 10.1128/AEM.69.1.399-407.2003

Figure Lengend Snippet: Summary of fermentation results a

Article Snippet: Broth cultures were grown in M9 medium containing either 1% glucose (tube experiments and seed cultures) or 5% glucose (pH-controlled fermentors). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains W3110 Wild type ATCC 27325 TOP10F′ lacI q (episome) Invitrogen DH5α Δ lacZ M15 recA Bethesda Research Laboratory S17-1 thi pro recA hsdR RP4-2- tet ::Mu aphA ::Tn Z λ pir 40 BW25113 lacI q rrnB Δ lacZ hsdR Δ araBAD Δ rhaBAD 16 AH218 Δ( focA-pflB ):: FRT-kan-FRT This study SE1706 Δ frdBC zid ::Tn 10 34 TC20 Δ adhE :: FRT-tet-FRT This study SZ31 W3110, Δ( focA-pflB ):: FRT-kan-FRT P1(AH218) × W3110 This study SZ32 W3110, Δ( focA-pflB ):: FRT This study SZ34 W3110, Δ( frdBC zid )::Tn 10 P1(SE1706) × W3110 This study SZ35 W3110, Δ( focA-pflB ):: FRT Δ frdBC zid ::Tn 10 P1(SE1706) × SZ32 This study SZ37 W3110, Δ frdBC This study SZ40 W3110, Δ( focA-pflB ):: FRT Δ frdBC This study SZ57 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT-tet-FRT P1(TC20) × SZ40 This study SZ58 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT This study SZ61 W3110, ackA :: FRT-tet-FRT This study SZ62 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT-tet-FRT P1(SZ61) × SZ58 This study SZ63 W3110, Δ focA-pflB :: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT This study Plasmids pCR2.1-TOPO bla kan , TOPO TA cloning vector Invitrogen pFT-A bla flp temperature-conditional replicon 37 pKD4 bla FRT-kan-FRT 16 pKD46 bla γ β exo (red recombinase), temperature-conditional replicon 16 pLOI2065 bla FRT-tet-FRT This study pLOI2224 kan , integration vector with conditional R6K replicon 31 pLOI2302 pUC19 containing Asc I linkers inserted into blunt Nde I and Sap I sites 46 pLOI2372 bla ackA This study pLOI2373 bla ackA :: FRT-tet-FRT This study pLOI2375 bla ackA :: FRT-tet-FRT conditional R6K replicon This study pLOI2803 bla kan Δ adhE :: FRT-tet-FRT This study Open in a separate window E. coli strains and plasmids used in this study Genetic methods.

Techniques:

Journal:

Article Title: Production of Optically Pure d -Lactic Acid in Mineral Salts Medium by Metabolically Engineered Escherichia coli W3110 †

doi: 10.1128/AEM.69.1.399-407.2003

Figure Lengend Snippet: HPLC profiles (refractive index monitor) from broth samples of W3110, SZ40, and SZ58 cultures at the end of fermentation. The two initial peaks that are not labeled are the inorganic components in M9 medium. The remaining peaks are identified by compound and retention time.

Article Snippet: Broth cultures were grown in M9 medium containing either 1% glucose (tube experiments and seed cultures) or 5% glucose (pH-controlled fermentors). table ft1 table-wrap mode="anchored" t5 TABLE 1. caption a7 Strain or plasmid Relevant characteristics Source or reference Strains W3110 Wild type ATCC 27325 TOP10F′ lacI q (episome) Invitrogen DH5α Δ lacZ M15 recA Bethesda Research Laboratory S17-1 thi pro recA hsdR RP4-2- tet ::Mu aphA ::Tn Z λ pir 40 BW25113 lacI q rrnB Δ lacZ hsdR Δ araBAD Δ rhaBAD 16 AH218 Δ( focA-pflB ):: FRT-kan-FRT This study SE1706 Δ frdBC zid ::Tn 10 34 TC20 Δ adhE :: FRT-tet-FRT This study SZ31 W3110, Δ( focA-pflB ):: FRT-kan-FRT P1(AH218) × W3110 This study SZ32 W3110, Δ( focA-pflB ):: FRT This study SZ34 W3110, Δ( frdBC zid )::Tn 10 P1(SE1706) × W3110 This study SZ35 W3110, Δ( focA-pflB ):: FRT Δ frdBC zid ::Tn 10 P1(SE1706) × SZ32 This study SZ37 W3110, Δ frdBC This study SZ40 W3110, Δ( focA-pflB ):: FRT Δ frdBC This study SZ57 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT-tet-FRT P1(TC20) × SZ40 This study SZ58 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT This study SZ61 W3110, ackA :: FRT-tet-FRT This study SZ62 W3110, Δ( focA-pflB ):: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT-tet-FRT P1(SZ61) × SZ58 This study SZ63 W3110, Δ focA-pflB :: FRT Δ frdBC Δ adhE :: FRT ackA :: FRT This study Plasmids pCR2.1-TOPO bla kan , TOPO TA cloning vector Invitrogen pFT-A bla flp temperature-conditional replicon 37 pKD4 bla FRT-kan-FRT 16 pKD46 bla γ β exo (red recombinase), temperature-conditional replicon 16 pLOI2065 bla FRT-tet-FRT This study pLOI2224 kan , integration vector with conditional R6K replicon 31 pLOI2302 pUC19 containing Asc I linkers inserted into blunt Nde I and Sap I sites 46 pLOI2372 bla ackA This study pLOI2373 bla ackA :: FRT-tet-FRT This study pLOI2375 bla ackA :: FRT-tet-FRT conditional R6K replicon This study pLOI2803 bla kan Δ adhE :: FRT-tet-FRT This study Open in a separate window E. coli strains and plasmids used in this study Genetic methods.

Techniques: Refractive Index, Labeling

Journal:

Article Title: Interaction between FliE and FlgB, a Proximal Rod Component of the Flagellar Basal Body of Salmonella

doi:

Figure Lengend Snippet: Strains and plasmids used in this study

Article Snippet: Luria-Bertani (LB) medium, soft tryptone agar plates, and M9-Casamino Acids medium are described in reference 14 . table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Strain or plasmid Relevant characteristics Source or reference E. coli strains XL1-Blue Recipient for cloning experiments Stratagene BL21(DE3)pLysS Overproduction of proteins from pET-based plasmid Novagen HMS174(DE3)pLysS Overproduction of proteins from pET-based plasmid Novagen Salmonella strains SJW1103 Wild-type strain for motility and chemotaxis 25 SJW1110 fliE -V99G This study MY7001 fliE -V99G flgB1 This study MY7002 fliE -V99G flgB2 This study SJW1190 Wild type This study SJW1191 flgB1 This study SJW1192 flgB2 This study SJW501 flgA ::Tn 10 This study SJW503 flgE ::Tn 10 This study SJW720 flgG ::Tn 10 This study SJW91 flhA ::Tn 10 This study SJW1572 Δ fliE-fliK 24 SJW1110- flgA fliE flgA This study SJW1110- flgG fliE flgG This study SJW1110- flhA fliE flhA This study SJW1525 flgB 19 SJW156 flgD 19 SJW1371 Δ fliE 19 Plasmids pET-FLAG-19b Cloning vector 2 pMM1001 pTrc99A/FliE This study pMM1006 pTrc99A/FliE-V99G This study pMM1003 pET19b/His-FLAG-FliE 15 pMM1005 pET19b/His-FLAG-FliE-V99G This study pMM1101 pTrc99A/FlgB This study pMM1106 pTrc99A/FlgB1 This study pMM1108 pTrc99A/FlgB2 This study pMM1102 pET19b/His-FlgB 15 pMM1105 pET19b/His-FlgB1 This study pMM1107 pET19b/His-FlgB2 This study pMM1202 pET19b/His-FlgC This study pMM1302 pET19b/His-FlgF This study pMM204 pET19b/His-FlgG This study pMM1601 pET19b/His-FlgE 15 pMM711 pET19b/His-FlgD 15 pMM1002 pET19b/His-FliE 15 pMM601 pET19b/His-MotA C 15 Open in a separate window Strains and plasmids used in this study Strain construction and mapping of suppressor mutations.

Techniques: Plasmid Preparation, Clone Assay, Chemotaxis Assay